Diagnosis of Enterocytozoon bieneusi by PCR in Stool Samples Eluted from Filter Paper Disks
2000; American Society for Microbiology; Volume: 7; Issue: 3 Linguagem: Inglês
10.1128/cdli.7.3.504-506.2000
ISSN1098-6588
AutoresSilvana Carnevale, Jorge Néstor Velásquez, Jorge H. Labbé, Agustín Víctor Chertcoff, Marta Cabrera, M. Rodríguez,
Tópico(s)Fecal contamination and water quality
ResumoABSTRACT We report a PCR-based assay for the detection of Enterocytozoon bieneusi . We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.
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