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Lack of association between the interferon-α signature and longitudinal changes in disease activity in systemic lupus erythematosus

2008; BMJ; Volume: 68; Issue: 9 Linguagem: Inglês

10.1136/ard.2008.093146

1468-2060

Carolina Landolt-Marticorena, Gabriel Bonventi, Ala Lubovich, C. William Ferguson, T Unnithan, Jinmei Su, Dafna D. Gladman, Murray B. Urowitz, Paul R. Fortin, Joan Wither,

Atherosclerosis and Cardiovascular Diseases

Objective: To study the longitudinal expression of interferon (IFN)-inducible genes in systemic lupus erythematosus (SLE) and determine their suitability as disease biomarkers. Methods: RNA was isolated from the peripheral blood of 94 patients with SLE and 11 controls and reverse transcribed into cDNA. The expression levels of five IFN-responsive genes ( LY6E, OAS1, IFIT1, ISG15 and MX1 ) were determined by quantitative PCR, normalised to GAPDH and summed to generate a global IFN score. Patients were followed longitudinally for a period of 3–12 months, and the association between disease activity, as measured by the SLE disease activity index (SLEDAI-2K), and other clinical and laboratory variables was examined. Results: The expression of all five IFN-responsive genes was significantly higher in patients with SLE than in controls. The expression of LY6E , OAS1 , IFIT1 and the global IFN score was associated with high disease activity. The global IFN score was also associated with active renal disease, a decreased C3, and the presence of anti-dsDNA or anti-RNA binding protein antibodies at a single point in time. However, there was a poor correlation between changes in this score and changes in disease activity, C3 or anti-dsDNA antibody levels in patients followed longitudinally. In most patients the levels of IFN-induced gene expression remained relatively stable over 3–12 months despite marked changes in disease activity. Nevertheless, in patients with low/moderate disease activity, those with high IFN scores had a more recent history of sustained high disease activity. Conclusion: The findings indicate that IFN-induced gene expression has limited clinical utility as a biomarker of acute changes in disease activity.