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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR

1999; Elsevier BV; Volume: 34; Issue: 2 Linguagem: Inglês

10.1016/s0732-8893(99)00021-8

ISSN

1879-0070

Autores

Elenice Lima de Castro Nunes, Kátia Regina Netto dos Santos, Pedro Juan José Mondino, Maria do Carmo de Freire Bastos, Márcia Giambiagi-deMarval,

Tópico(s)

Infective Endocarditis Diagnosis and Management

Resumo

The presence of the ileS-2 gene, responsible for mupirocin resistance, in clinical isolates of methicillin-resistant Staphylococcus aureus was determined by multiplex polymerase chain reaction. Three pairs of primers were used, which yielded specific fragments of femA (encoding a unique feature of S. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving technique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories.

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