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Role of the mesosalpinx and oviduct vasculature in the counter-current transfer of steroid hormones into the ovary

1982; Elsevier BV; Volume: 5; Issue: 1 Linguagem: Inglês

10.1016/0378-4320(82)90034-3

1873-2232

T Krzymowski, S. Stefańczyk, Marek Koziorowski, J. Czarnocki, T. Ruszczyk, R Nowicka,

Sperm and Testicular Function

On days 14–17 of the oestrous cycle, the ovary with its ovarian pedicle was isolated during laparatomy, placed on a water bath (40°C) under a stereomicroscope, protected from dessication and supplied with autologous arterial blood. 50 μCi of 3H-testosterone was infused (30 min) by cannula, the tip of which was positioned in the ovarian vein 1–2 cm from the ovary. Radioactivity was found in the blood taken from both the ovarian and the oviduct branches of the ovarian artery. The radioactivity of the ovarian venous effluent and testosterone capture by the ovary and ovarian tissues during and 30 min after testosterone infusion were determined. In subsequent experiments the mesosalpinx of an infused preparation (preparation A) closely covered a second isolated ovary (preparation B) placed nearby in a separate box and supplied with arterial blood. The level of radioactivity in preparation B was estimated in the arterial and venous blood, in samples of lymph and interstitial fluid, in the follicular fluid and in the ovarian and pedicle tissues. During the experiment 0.6% of the 3H-testosterone infused into preparation A was transferred through the mesosalpinx vasculature into the ovary and ovarian pedicle of preparation B. This indicates the existence of a still unknown way for penetration of steroid hormones from the ovarian pedicle through the mesosalpinx vasculature and the ovarian surface into the tissues of the ovary.