Comparison between RAST and Pharmacia CAP system: A new automated specific IgE assay
1990; Elsevier BV; Volume: 85; Issue: 6 Linguagem: Inglês
10.1016/0091-6749(90)90048-9
ISSN1097-6825
AutoresJean Bousquet, Pascal Chanez, I. Chanal, F. B. Michel,
Tópico(s)Contact Dermatitis and Allergies
ResumoRAST represents the standard technique to titrate serum-spectjic IgE and was found to have u higher efjiciency in the diagnosis of &E-mediated allergy than other in vitro tests.Pharmacia CAP system (CAP) is a new solid-phase immunoassay, fUray automated, used for the titration oj specific IgE antibodies.Results are listed in kilounits per liter, equilibrated against the World Health Organization standard for IgE.RAST and CAP were compared in IO6 unselected patients (6 to 59 years) characterized by a detailed clinical history and skin prick tests with standardized allergen extracts.IgE to cat, Dermalophagoides pteronyssinus, Aiternaria, orchard grass, olive, and Parietaria pollen were tested; 470 tests were run.The specificity, sensitivity, and eflciency of both in vitro tests ranged from 85.5% to 100% except for olive pollen, in which the sensitivity of both in vitro tests was low (68.2% for the new test and 63.6% for RAST).Except for orchard-grass pollen, the sensitivity and specifiity of CAP were better than that of RAST.There was a highly significant correlation between both tests (r range, between 0.864 to 0.987).CAP competes favorably with RAST and has the advantage of being automated and eliciting results in kilounits per liter.(J ALLERGY CLIN IMMJNOL 1990;85:1039-43.j Specific serum IgE is widely used in the diagnosis of IgE-mediated allergic diseases.Although several techniques have been proposed within the past 10 years, RAST represents the standard technique.In-
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