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Isolation of a human prostate carcinoma cell line (DU 145)

1978; Wiley; Volume: 21; Issue: 3 Linguagem: Inglês

10.1002/ijc.2910210305

1097-0215

Kenneth R. Stone, Don D. Mickey, Heidi Wunderli, George H. Mickey, David F. Paulson,

Molecular Biology Techniques and Applications

Abstract A long‐term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.