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Application of protein-fragment complementation assays in cell biology

2007; Future Science Ltd; Volume: 42; Issue: 2 Linguagem: Inglês

10.2144/000112396

1940-9818

Ingrid Remy, Stephen W. Michnick,

Advanced Proteomics Techniques and Applications

We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.