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Advanced methods of microscope control using μManager software

2014; POL Scientific; Volume: 1; Issue: 2 Linguagem: Inglês

10.14440/jbm.2014.36

2326-9901

Arthur Edelstein, Mark A. Tsuchida, Nenad Amodaj, Henry Pinkard, Ronald D. Vale, Nico Stuurman,

Advanced Electron Microscopy Techniques and Applications

μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.