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A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis

2008; Oxford University Press; Volume: 198; Issue: 10 Linguagem: Inglês

10.1086/592509

1537-6613

Stijn Deborggraeve, Thierry Laurent, Diego A. Espinosa, Gert Van der Auwera, Margaret Mbuchi, Monique Wasunna, Sayda El‐Safi, Ahmed Almustafa Al‐Basheer, Jorge Arévalo, César Miranda‐Verástegui, T. Leclipteux, Pascal Mertens, Jean‐Claude Dujardin, Piet Herdewijn, Philippe Büscher,

Trypanosoma species research and implications

Background. Definite diagnosis of Leishmania infections is based on demonstration of the parasite by microscopic analysis of tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling.