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Nitrogen recycling in the pea aphid ( Acyrthosiphon pisum ) symbiosis

1992; Royal Society; Volume: 250; Issue: 1328 Linguagem: Inglês

10.1098/rspb.1992.0138

1471-2954

Lynne Whitehead, T. L. Wilkinson, Angela E. Douglas,

Plant Parasitism and Resistance

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Whitehead L. F. , Wilkinson T. L. and Douglas A. E. 1992Nitrogen recycling in the pea aphid (Acyrthosiphon pisum) symbiosisProc. R. Soc. Lond. B.250115–117http://doi.org/10.1098/rspb.1992.0138SectionRestricted accessArticleNitrogen recycling in the pea aphid (Acyrthosiphon pisum) symbiosis L. F. Whitehead Google Scholar Find this author on PubMed Search for more papers by this author , T. L. Wilkinson Google Scholar Find this author on PubMed Search for more papers by this author and A. E. Douglas Google Scholar Find this author on PubMed Search for more papers by this author L. F. Whitehead Google Scholar Find this author on PubMed , T. L. Wilkinson Google Scholar Find this author on PubMed and A. E. Douglas Google Scholar Find this author on PubMed Published:23 November 1992https://doi.org/10.1098/rspb.1992.0138AbstractTwo lines of evidence suggest that the symbiotic bacteria in the pea aphid Acyrthosiphon pisum can assimilate ammonia. First, bacteria isolated from the aphids accumulated exogenous methylamine, an analogue of ammonia, and the bacterial transport system displayed saturation kinetics characteristic of carrier-mediated transport (apparent Km 50 µm; Vmax 2 nmol (mg protein)-1 min-1). Second, the concentration of ammonia in the honeydew of aphids was significantly increased when the bacteria were disrupted by antibiotics (chlortetracycline and rifampicin). Taken with previously published results, these data suggest that the bacteria in aphids recycle aphid waste ammonia to essential amino acids, which are made available to the aphid tissues. This nutritional interaction between aphids and their bacteria is linked to the very high ratio of non-essential : essential amino acids in phloem sap, the usual diet of aphids.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Bar‐Shmuel N, Behar A and Segoli M (2019) What do we know about biological nitrogen fixation in insects? Evidence and implications for the insect and the ecosystem, Insect Science, 10.1111/1744-7917.12697, 27:3, (392-403), Online publication date: 1-Jun-2020. Singh S, Arya S, Kaur G, Saxena G and Verma P (2019) Role of Endosymbionts in Nutritional Uptake of Sap Sucking Insects Molecular Approaches in Plant Biology and Environmental Challenges, 10.1007/978-981-15-0690-1_22, (487-499), . Paulson A, von Aderkas P and Perlman S (2014) Bacterial associates of seed-parasitic wasps (Hymenoptera: Megastigmus), BMC Microbiology, 10.1186/s12866-014-0224-4, 14:1, Online publication date: 1-Dec-2014. Macdonald S, Lin G, Russell C, Thomas G and Douglas A (2012) The central role of the host cell in symbiotic nitrogen metabolism, Proceedings of the Royal Society B: Biological Sciences, 279:1740, (2965-2973), Online publication date: 7-Aug-2012. Hansen A and Moran N (2011) Aphid genome expression reveals host–symbiont cooperation in the production of amino acids, Proceedings of the National Academy of Sciences, 10.1073/pnas.1013465108, 108:7, (2849-2854), Online publication date: 15-Feb-2011. 10.1007/BF00194774, CrossRef Listing of Deleted DOIs, 10.1007/BF00194774 Ramsey J, MacDonald S, Jander G, Nakabachi A, Thomas G and Douglas A (2010) Genomic evidence for complementary purine metabolism in the pea aphid, Acyrthosiphon pisum, and its symbiotic bacterium Buchnera aphidicola, Insect Molecular Biology, 10.1111/j.1365-2583.2009.00945.x, 19, (241-248) Janson E, Stireman J, Singer M and Abbot P (2008) PHYTOPHAGOUS INSECT–MICROBE MUTUALISMS AND ADAPTIVE EVOLUTIONARY DIVERSIFICATION, Evolution, 10.1111/j.1558-5646.2008.00348.x, 62:5, (997-1012), Online publication date: 1-May-2008. Douglas A (2003) The Nutritional Physiology of Aphids , 10.1016/S0065-2806(03)31002-1, (73-140), . Bernays E and Klein B (2002) Quantifying the symbiont contribution to essential amino acids in aphids: the importance of tryptophan for Uroleucon ambrosiae, Physiological Entomology, 10.1046/j.1365-3032.2002.00297.x, 27:4, (275-284), Online publication date: 1-Dec-2002. Wilkinson T (1998) The elimination of intracellular microorganisms from insects: an analysis of antibiotic-treatment in the pea aphid (Acyrthosiphon pisum), Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 10.1016/S1095-6433(98)00013-0, 119:4, (871-881), Online publication date: 1-Apr-1998. Douglas A (1998) Nutritional Interactions in Insect-Microbial Symbioses: Aphids and Their Symbiotic Bacteria Buchnera , Annual Review of Entomology, 10.1146/annurev.ento.43.1.17, 43:1, (17-37), Online publication date: 1-Jan-1998. Hirayama C, Konno K and Shinbo H (1997) The pathway of ammonia assimilation in the silkworm, Bombyx mori, Journal of Insect Physiology, 10.1016/S0022-1910(97)00045-0, 43:10, (959-964), Online publication date: 1-Oct-1997. Wilkinson T and Douglas A (2011) The impact of aposymbiosis on amino acid metabolism of pea aphids, Entomologia Experimentalis et Applicata, 10.1111/j.1570-7458.1996.tb00935.x, 80:1, (279-282), Online publication date: 1-Jul-1996. Wilkinson T and Douglas A (1996) The impact of aposymbiosis on amino acid metabolism of pea aphids Proceedings of the 9th International Symposium on Insect-Plant Relationships, 10.1007/978-94-009-1720-0_63, (279-282), . Dunn A, Hatcher M, Terry R and Tofts C (2009) Evolutionary ecology of vertically transmitted parasites: transovarial transmission of a microsporidian sex ratio distorter in Gammarus duebeni , Parasitology, 10.1017/S0031182000075843, 111:S1, (S91-S109), Online publication date: 1-Jan-1995. ABISGOLD J, SIMPSON S and DOUGLAS A (1994) Nutrient regulation in the pea aphid Acyrthosiphon pisum: application of a novel geometric framework to sugar and amino acid consumption, Physiological Entomology, 10.1111/j.1365-3032.1994.tb01081.x, 19:2, (95-102), Online publication date: 1-Jun-1994. Whitehead L and Douglas A (1997) Populations of symbiotic bacteria in the parthenogenetic pea aphid (Acyrthosiphon pisum) symbiosis, Proceedings of the Royal Society of London. Series B: Biological Sciences, 254:1339, (29-32), Online publication date: 22-Oct-1993. This Issue23 November 1992Volume 250Issue 1328 Article InformationDOI:https://doi.org/10.1098/rspb.1992.0138Published by:Royal SocietyPrint ISSN:0962-8452Online ISSN:1471-2954History: Manuscript received21/07/1992Manuscript accepted17/09/1992Published online01/01/1997Published in print23/11/1992 License:Scanned images copyright © 2017, Royal Society Citations and impact Large datasets are available through Proceedings B's partnership with Dryad