Artigo Revisado por pares

Three-dimensional microscopy with phase-shifting digital holography

1998; Optica Publishing Group; Volume: 23; Issue: 15 Linguagem: Inglês

10.1364/ol.23.001221

ISSN

1539-4794

Autores

Tong Zhang, Ichirou Yamaguchi,

Tópico(s)

Advanced Optical Imaging Technologies

Resumo

We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

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