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Loss of Werner syndrome protein function promotes aberrant mitotic recombination

2001; Cold Spring Harbor Laboratory Press; Volume: 15; Issue: 8 Linguagem: Inglês

10.1101/gad.877001

1549-5477

P.R. Prince, Mary J. Emond, Raymond J. Monnat,

Carcinogens and Genotoxicity Assessment

The chromosome 8p11–12 Werner syndrome ( WRN ) locus encodes a RecQ helicase protein of unknown function that possesses both 3′ → 5′ helicase and 3′ → 5′ exonuclease activities. We show that WRN cell lines display a marked reduction in cell proliferation following mitotic recombination, and generate few viable gene conversion-type recombinants. These findings indicate that WRN plays a role in mitotic recombination, and that a loss of WRN function may promote genetic instability and disease via recombination-initiated mitotic arrest, cell death, or gene rearrangement.