Produção Nacional
Revisado por pares
C-Phycocyanin protects SH-SY5Y cells from oxidative injury, rat retina from transient ischemia and rat brain mitochondria from Ca2+/phosphate-induced impairment
2012; Elsevier BV; Volume: 89; Issue: 5-6 Linguagem: Inglês
10.1016/j.brainresbull.2012.08.011
ISSN1873-2747
AutoresJavier Marín‐Prida, Giselle Pentón‐Rol, Fernando Postalli Rodrigues, Luciane C. Alberici, Karina Stringhetta, Andréia Machado Leopoldino, Zeki Naal, Ana Cristina Morseli Polizello, Alexey Llopiz, Marcela N. Rosa, José Luiz Liberato, Wagner Ferreira dos Santos, Sérgio Akira Uyemura, Eduardo Pentón‐Arias, Carlos Curti, Gilberto L. Pardo‐Andreu,
Tópico(s)Neurological Disease Mechanisms and Treatments
ResumoOxidative stress and mitochondrial impairment are essential in the ischemic stroke cascade and eventually lead to tissue injury. C-Phycocyanin (C-PC) has previously been shown to have strong antioxidant and neuroprotective actions. In the present study, we assessed the effects of C-PC on oxidative injury induced by tert-butylhydroperoxide (t-BOOH) in SH-SY5Y neuronal cells, on transient ischemia in rat retinas, and in the calcium/phosphate-induced impairment of isolated rat brain mitochondria (RBM). In SH-SY5Y cells, t-BOOH induced a significant reduction of cell viability as assessed by an MTT assay, and the reduction was effectively prevented by treatment with C-PC in the low micromolar concentration range. Transient ischemia in rat retinas was induced by increasing the intraocular pressure to 120 mmHg for 45 min, which was followed by 15 min of reperfusion. This event resulted in a cell density reduction to lower than 50% in the inner nuclear layer (INL), which was significantly prevented by the intraocular pre-treatment with C-PC for 15 min. In the RBM exposed to 3 mM phosphate and/or 100 μM Ca2+, C-PC prevented in the low micromolar concentration range, the mitochondrial permeability transition as assessed by mitochondrial swelling, the membrane potential dissipation, the increase of reactive oxygen species levels and the release of the pro-apoptotic cytochrome c. In addition, C-PC displayed a strong inhibitory effect against an electrochemically-generated Fenton reaction. Therefore, C-PC is a potential neuroprotective agent against ischemic stroke, resulting in reduced neuronal oxidative injury and the protection of mitochondria from impairment.
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