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Restricted rosette formation with an EAC reagent prepared with serum of C3H/HeJ mice

1980; Elsevier BV; Volume: 51; Issue: 2 Linguagem: Inglês

10.1016/0008-8749(80)90271-3

1090-2163

Johannes van Dijk, D. van Heuven-Nolsen, Pieternel M. Rademaker, Nanne Bloksma, J M Willers,

Prostate Cancer Treatment and Research

The EAC generating capacities of fresh and stored BALBic, Swiss, C3HeB/FeJ.and C3HIHeJ mouse set-a were investigated in a rosette test using spleen cells of each of the serum donors as indicator cells.With BALBic spleen cells a decreased ability of both stored and fresh C3HIHeJ serum to generate functional EAC could be registered, whereas with C3HeBIFeJ spleen cells such was the case only with stored serum.Swiss spleen cells did not discriminate between the capacities of the four sera and with C3H spleen cells both C3H sera tended to be less effective than BALBic and Swiss serum.This indicates that C3HIHeJ serum is able to generate EAC but with a somewhat different disposition of cell-bound C molecules.Our results suggest that the "defect" in C3HIHeJ serum is localized in C,, C:,, or rather in some C-associated inhibiting system as Cd-binding protein or the C:,,, inactivating system.A possible relationship of the "defective" serum factor and the defective Lps gene product is suggested.