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Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae

1999; Oxford University Press; Volume: 174; Issue: 1 Linguagem: Inglês

10.1111/j.1574-6968.1999.tb13551.x

1574-6968

Marcel T. Flikweert, Martin E. de Swaaf, Johannes P. van Dijken, Jack T. Pronk,

Microbial Metabolic Engineering and Bioproduction

Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc−S. cerevisiae does not exhibit prolonged growth on glucose. Pdc− strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.