Portal de Periódicos da CAPES

Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope

1993; American Society for Microbiology; Volume: 67; Issue: 8 Linguagem: Inglês

10.1128/jvi.67.8.4742-4749.1993

1098-5514

Stefania Lombardi, Carlo Garzelli, Corinna La Rosa, Lucia Zaccaro, S. Specter, G Malvaldi, F Tozzini, Fulvio Esposito, M Bendinelli,

vaccines and immunoinformatics approaches

Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.