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Transcriptional profiling of “guided bone regeneration” in a critical-size calvarial defect

2011; Wiley; Volume: 22; Issue: 4 Linguagem: Inglês

10.1111/j.1600-0501.2010.02104.x

1600-0501

Sašo Ivanovski, Stephen Hamlet, M. Retzepi, Ivan Wall, Nikolaos Donos,

TGF-β signaling in diseases

Clinical Oral Implants ResearchVolume 22, Issue 4 p. 382-389 Transcriptional profiling of “guided bone regeneration” in a critical-size calvarial defect S. Ivanovski, S. Ivanovski School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Gold Coast Campus, Qld, AustraliaSearch for more papers by this authorS. Hamlet, S. Hamlet School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Gold Coast Campus, Qld, AustraliaSearch for more papers by this authorM. Retzepi, M. Retzepi Periodontology Unit, UCL Eastman Dental Institute, London, UKSearch for more papers by this authorI. Wall, I. Wall Regenerative Medicine Bioprocessing Unit, UCL Advanced Centre for Biochemical Engineering, London, UKSearch for more papers by this authorN. Donos, N. Donos Periodontology Unit, UCL Eastman Dental Institute, London, UKSearch for more papers by this author S. Ivanovski, S. Ivanovski School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Gold Coast Campus, Qld, AustraliaSearch for more papers by this authorS. Hamlet, S. Hamlet School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Gold Coast Campus, Qld, AustraliaSearch for more papers by this authorM. Retzepi, M. Retzepi Periodontology Unit, UCL Eastman Dental Institute, London, UKSearch for more papers by this authorI. Wall, I. Wall Regenerative Medicine Bioprocessing Unit, UCL Advanced Centre for Biochemical Engineering, London, UKSearch for more papers by this authorN. Donos, N. Donos Periodontology Unit, UCL Eastman Dental Institute, London, UKSearch for more papers by this author First published: 09 March 2011 https://doi.org/10.1111/j.1600-0501.2010.02104.xCitations: 51 Corresponding author: Professor Saso Ivanovski School of Dentistry and Oral HealthCentre for Medicine and Oral HealthGriffith UniversityGold Coast CampusQld 4222AustraliaTel.: +61 7 5678 0741Fax: +61 7 5678 0708e-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Objectives: Guided bone regeneration (GBR) is a commonly utilized surgical technique in the craniofacial region. The transcriptional mechanisms associated with this type of bone regeneration are not well understood. The aim of this study was to characterize the transcriptome associated with GBR of a critical-size calvarial defect in the rat. Material and methods: Critical-size calvarial defects were created in six Wistar strain rats and treated according to the principles of GBR. The tissue filling the regenerating defect was harvested at 7 and 14 days. Total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 7 and 14. Results: Gene ontology (GO) analysis of the genes up-regulated at day 7 showed that immature wound healing-related mechanisms, such as protein metabolism and cell proliferation, were up-regulated at this time point. Furthermore, the immuno-inflammatory process was also up-regulated at the earlier time point. In contrast, by day 14, GO groups consistent with wound maturation, such as extracellular matrix formation, anatomical structure development and cell differentiation, were up-regulated. Furthermore, the functionally important GO categories of skeletal development, ossification and bone mineralization were up-regulated at day 14. Genes of interest that belonged to this group and were up-regulated at day 14 included growth and differentiation factors (Bmp2, Bmp3, Tgfb3), extracellular matrix proteins (osteocalcin, osteomodulin, stenniocalcin 1) and transcription factors (Runx2, Sox6, Satb2). Furthermore, a number of genes associated with Tgfβ/Bmp and Wnt signalling were also up-regulated. Besides skeletogenesis, genes associated with angiogenesis and neurogenesis were also up-regulated at day 14. Conclusions: The transcriptome associated with a maturing GBR-treated craniofacial bone defect is characterized by the down-regulation of the immuno-inflammatory response and up-regulation of skeletogeneis-, angiogenesis- and neurogenesis-associated genes. The Tgfβ/Bmp and Wnt signalling pathways play an important role in the regenerative process. To cite this article: Ivanovski S, Hamlet S, Retzepi M, Wall I, Donos N. Transcriptional profiling of “guided bone regeneration” in a critical size calvarial defect.Clin. Oral Impl. Res. 22, 2011; 382–389. References Axelrad, T.W. & Einhorn, T.A. (2009) Bone morphogenetic proteins in orthopaedic surgery. Cytokine and Growth Factor Reviews 20: 481–488. 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