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Studies on the orientation of brush-border membrane vesicles

1978; Portland Press; Volume: 172; Issue: 1 Linguagem: Inglês

10.1042/bj1720057

1470-8728

Winfried Haase, A Schäfer, Heini Murer, R. Kinne,

Infant Nutrition and Health

Orientation of rat renal and intestinal brush-border membrane vesicles was studied with two independent methods: electron-microscopic freeze-fracture technique and immunological methods. With the freeze-fracture technique a distinct asymmetric distribution of particles on the two membrane fracture faces was demonstrated; this was used as a criterion for orientation of the isolated membrane vesicles. For the immunological approach the accessibility or inaccessibility of aminopeptidase M localized on the outer surface of the cell membrane to antibodies was used. With both methods we showed that the brush-border membrane vesicles isolated from rat kidney cortex and from rat small intestine for transport studies are predominantly orientated right-side out.