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BIBTEX RIS

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

2013; Oxford University Press; Volume: 194; Issue: 4 Linguagem: Inglês

10.1534/genetics.113.152710

ISSN

1943-2631

Autores

Scott J Gratz, Alexander M Cummings, Jennifer N. Nguyen, Danielle C. Hamm, Laura K. Donohue, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles,

Tópico(s)

Insect symbiosis and bacterial influences

Resumo

Abstract We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

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