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DETERMINATION OF ACID PHOSPHATASE ACTIVITY IN TISSUE HOMOGENATES

1966; Canadian Science Publishing; Volume: 44; Issue: 6 Linguagem: Inglês

10.1139/o66-093

0008-4018

John P. Manning, Arthur L. Babson, M. C. Butler, Sara F. Priester,

Metabolism and Genetic Disorders

A simple and accurate method is described for the quantitative determination of acid phosphatase in tissue homogenates, utilizing stable buffered sodium α-naphthyl acid phosphate tablets as substrate, and the monocoupler Fast Red Salt B as the color developer. This technique eliminates deproteinization and ethyl acetate extraction, and produces a highly colored blue azo dye for spectrophotometry. Of the rat organs tested, the spleen had the highest acid phosphatase activity, then kidney, small intestine, liver, lung, and heart, in that order.