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A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping

1993; Cell Press; Volume: 73; Issue: 1 Linguagem: Inglês

10.1016/0092-8674(93)90165-m

1097-4172

Toru Mizukami, William I. Chang, Igor Garkavtsev, Nancy Kaplan, D. Rachel Lombardi, Tomohiro Matsumoto, Osami Niwa, Asako Kounosu, Mitsuhiro Yanagida, Thomas G. Marr, David Beach,

Genomics and Phylogenetic Studies

We present the application of a nonrandom sequencetagged site (STS) content detection method in mapping an entire genome, that of fission yeast. The novelty of our strategy is in the use of STS probes made from both ends of cosmid clones, selected on the basis of “sample without replacement” (only library clones that show no previous positive hybridization are selected and made into probes). We developed powerful techniques, based on consistency analysis, for error detection and contig assembly. In addition, we probed our library with genetically mapped markers and Notl or Stil linking clones, thereby anchoring contigs onto chromosomes. Our map contains more than 1000 sites, including genes (most were previously unmapped), occurrences of known repetitive elements, and Notl-Stil restriction sites.