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Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.

2009; Microbiology Society; Volume: 60; Issue: 10 Linguagem: Inglês

10.1099/ijs.0.016543-0

1466-5034

Midori Kurahashi, Yukiyo Fukunaga, Yayoi Sakiyama, Shigeaki Harayama, Akira Yokota,

Coral and Marine Ecosystems Studies

A tangerine-coloured, Gram-positive actinobacterial strain, designated F10 T , was isolated from the abdominal epidermis of a sea cucumber, Holothuria edulis , collected in seawater off the coast of Japan. A 16S rRNA gene sequence analysis indicated that strain F10 T was a member of the class Actinobacteria and was most closely related to Nitriliruptor alkaliphilus ANL-iso2 T (87.4 % sequence similarity). Phylogenetic analyses showed that strain F10 T represented a novel, deep-rooted, and distinct phylogenetic lineage within the class Actinobacteria and clustered with N. alkaliphilus and uncultured bacteria. The organism had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and rhamnose and galactose as the diagnostic cell-wall sugars. Strain F10 T contained C 16 : 1 ω 7 c , C 16 : 0 and C 17 : 1 ω 8 c as the major cellular fatty acids. The predominant isoprenoid quinone was MK-9 (H 4 ). The G+C content of the DNA was 68.3 mol%. Based on data from the current polyphasic study, it is proposed that the new marine isolate be placed in a novel genus and be considered a novel species designated Euzebya tangerina gen. nov., sp. nov. within the new family, order and subclass Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov. in the class Actinobacteria . The type strain of Euzebya tangerina is F10 T (=NBRC 105439 T =KCTC 19736 T ).