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Sensory And Sympathetic Innervation Of The Vertebral Endplate In Patients With Degenerative Disc Disease

1997; British Editorial Society of Bone & Joint Surgery; Volume: 79; Issue: 1 Linguagem: Inglês

10.1302/0301-620x.79b1.6814

2044-5377

Mark F. Brown, Mika Hukkanen, I. D. McCarthy, Daniel Redfern, J. Batten, HV Crock, Sean Hughes, J. M. Polak,

Pain Mechanisms and Treatments

We obtained intervertebral discs with cartilage endplates and underlying cancellous bone at operation from patients with degenerative disc disease and then used immunohistochemical techniques to localise the nerves and nerve endings in the specimens. We used antibodies for the ubiquitous neuronal protein gene product 9.5 (PGP 9.5). Immunoreactivity to neuropeptide Y was used to identify autonomic nerves and calcitonin gene-related peptide (CGRP) and substance P to identify sensory nerves. Blood vessels were identified by immunoreactivity with platelet-endothelial cell-adhesion molecule (CD31; PECAM).