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Effect of Oral Kohki Tea on Bladder Dysfunction Induced by Severe Partial Outlet Obstruction

2002; Lippincott Williams & Wilkins; Volume: 167; Issue: 5 Linguagem: Inglês

10.1016/s0022-5347(05)65139-7

1527-3792

Robert M. Levin, Yoshihito Kawashima, Robert E. Leggett, Catherine Whitbeck, Patrick Horan, Kenji Mizutani,

Phytochemistry and Biological Activities

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 May 2002Effect of Oral Kohki Tea on Bladder Dysfunction Induced by Severe Partial Outlet Obstruction ROBERT M. LEVIN, YOSHIHITO KAWASHIMA, ROBERT E. LEGGETT, CATHERINE WHITBECK, PATRICK HORAN, and KENJI MIZUTANI ROBERT M. LEVINROBERT M. LEVIN , YOSHIHITO KAWASHIMAYOSHIHITO KAWASHIMA , ROBERT E. LEGGETTROBERT E. LEGGETT , CATHERINE WHITBECKCATHERINE WHITBECK , PATRICK HORANPATRICK HORAN , and KENJI MIZUTANIKENJI MIZUTANI View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65139-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Extracts of the leaves of Engelhardtia chrysolepis, a subtropical plant that grows wild in southern China, have been used medicinally in east Asia for hundreds of years. A standard extract named Kohki tea (Maruzen Pharmaceuticals, Onomichi City, Japan) is sold over the counter in Japan as a sweet tea shown to confer many beneficial effects on general health and well-being. The tea contains strong antioxidants, including several dihydroflavonol glycosides. The results of previous studies show that natural products with antioxidant activities provide protective effects on the bladder of rabbits with partial outlet obstruction. We determined in vivo and in vitro whether oral pretreatment of rabbits with Kohki tea protects the bladder from dysfunction induced by partial outlet obstruction. Materials and Methods: A total of 28 New Zealand White rabbits were separated into 4 groups of 7 each. Rabbits in groups 1 and 2 were treated by gavage with 100 mg./kg. Kohki tea daily in distilled water, while those in groups 3 and 4 were given distilled water. After 4 weeks of daily oral administration each rabbit was sedated, the bladder was catheterized and cystometry was performed at a filling rate of 1 ml. per minute. At the completion of cystometry the rabbits were immediately anesthetized. Moderate outlet obstruction was created in groups 1 and 3, and sham surgery was performed in groups 2 and 4. Treatment was continued for an additional 4 weeks, when each rabbit was sedated and cystometry was repeated. After cystometry the bladder was exposed through a midline incision, excised, weighed and 4 strips of bladder body were cut for contractility studies. The balance of the bladder was separated between smooth muscle and mucosa by blunt dissection, frozen in liquid nitrogen and stored at −70C for biochemical analyses. Results: Partial outlet obstruction stimulated similar increases in the bladder weight of all obstructed rabbits. Partial outlet obstruction resulted in a significant decrease in bladder compliance in all obstructed animals. However, the bladder of obstructed rabbits given Kohki tea were significantly more compliant than those given water. Voiding pressures in the control group and the obstructed group given distilled water were approximately equal, while obstructed rabbits given Kohki tea showed significantly higher maximal voiding pressure. The contractile responses to all forms of stimulation were reduced by obstruction to a significantly greater degree in the rabbits not given tea than in those given tea. Sarcoplasmic reticulum Ca2+-adenosine triphosphatase enzyme activity of the bladder was significantly reduced in obstructed rabbits given vehicle but activity was not reduced in obstructed rabbits given Kohki tea. Conclusions: Kohki tea had a significant protective effect on bladder function, contractile responses and bladder biochemistry in rabbits with moderate to severe partial outlet obstruction. References 1 : Benign Prostatic Hypertrophy. New York: Springer-Verlag1983. Google Scholar 2 : The physical basis of obstructive uropathy. In: Benign Prostatic Hypertrophy.. Edited by . New York: Springer-Verlag1983. Google Scholar 3 : Benign prostatic hyperplasia. In: Adult and Pediatric Urology.. Edited by . Chicago: Yearbook Medical1987. Google Scholar 4 : Acute biochemical and functional alterations in the partially obstructed rabbit urinary bladder. J Urol1986; 136: 1324. Link, Google Scholar 5 : The functional effect of mild outlet obstruction on the rabbit urinary bladder. J Urol1988; 140: 880. Link, Google Scholar 6 : The guinea pig as a model of gradual urethral obstruction. J Urol1991; 145: 854. Link, Google Scholar 7 : The effect of bladder outlet obstruction on tissue oxygen tension and blood flow in the pig bladder. BJU Int2000; 9: 1109. Google Scholar 8 : Canine bladder blood flow and oxygenation: changes induced by filling, contraction and outlet obstruction. J Urol1996; 155: 1459. Link, Google Scholar 9 : Blood flow of the urinary bladder: effects of outlet obstruction and correlation with bioenergetic metabolism. Neurourol Urodyn1995; 14: 285. Google Scholar 10 : Experimental models of bladder obstruction. In: Prostatic Disease.. Edited by . Philadelphia: W. B. Saunders1999: 169. Google Scholar 11 : Contractility and phenotype transitions in serosal thickening of obstructed rabbit bladder. J Applied Physiol1995; 78: 1432. Google Scholar 12 : The effect of bladder outflow obstruction on detrusor blood flow changes during the voiding cycle in conscious pigs. J Urol2001; 165: 245. Link, Google Scholar 13 : Oxidative stress in mitochondria: its relationship to cellular Ca2+ homeostasis, cell death, proliferation, and differentiation. Chem Biol Interact1991; 77: 1. Google Scholar 14 : Oxidants in mitochondria: from physiology to diseases. Biochim Biophys Acta1995; 1271: 67. Google Scholar 15 : An evaluation of the role of mitochondria in neurodegenerative diseases: mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration. Brain Res Rev1999; 29: 1. Google Scholar 16 : Correlation of ischemia/reperfusion and partial outlet obstruction induced spectrin proteolysis by calpain with contractile dysfunction in the rabbit bladder. Urology1997; 49: 293. Google Scholar 17 : Calpain: new perspectives in molecular diversity and physiological-pathological involvement. FASEB J1994; 8: 814. Google Scholar 18 : Fatty acid profiles in normal and obstructed rabbit bladder smooth muscle and mucosa. Neurourol Urodyn1999; 18: 697. Google Scholar 19 : Mechanisms of phospholipase A2 activation and neuronal injury. Ann N Y Acad Sci1993; 679: 110. Google Scholar 20 : Oxygen free radical-induced lipid peroxidation in overdistention of the rabbit urinary bladders. Neurourol Urodyn1995; 14: 553. Google Scholar 21 : Correlation of lipid peroxidation with energetic metabolism and contractile function in overdistention of the rabbit urinary bladder. Neurourol Urodyn1996; 15: 426. Google Scholar 22 : A scientific basis for the therapeutic effects of Pygium africanum and Serenoa repens. Urol Res2000; 28: 201. Google Scholar 23 : Effect of oral Tadenan treatment on rabbit bladder structure and function after bladder outlet obstruction. J Urol2002; 167: 2253. Abstract, Google Scholar 24 : Antioxidative substances in leaves of Polygonum Hudopiper. J Agric Food Chem1992; 40: 1349. Google Scholar 25 : Effect of astilbin in tea processed from leaves of Engelhardtia chrysolepis on the serum and liver lipid concentrations and on the erythrocyte and liver antioxidative enzyme activities of rats. Biosci Biotech Biochem1996; 60: 513. Google Scholar 26 : Enhancement of the vanadate-stimulated release of lipoprotein lipase activity by astilbin from the leaves of Engelhardtia chrysolepis. Biol Pharmacol Bull1998; 21: 517. Google Scholar 27 : Inhibition of aldose reductase and sorbitol accumulation by astilbin and taxifolin dihydroflavonols in Engelhardtia chrysolepis. Biosci Biotechn Biochem1997; 61: 651. Google Scholar 28 : Inhibition of aldose reductase by dihydroflavonols in Engelhardtia chrysolepis and effects on other enzymes. Experientia1996; 52: 564. Google Scholar 29 : Protection against oxidative damage by dihydroflavonols in Engelhardtia chrysolepis. Biosci Biotechnol Biochem1996; 60: 945. Google Scholar 30 : Mechanisms of bladder smooth muscle hypertrophy and decompensation: lessons from normal development and the response to outlet obstruction. In: The Bladder: Basic and Clinical Research.. Edited by . New York: Plenum1996. Google Scholar 31 : Pharmacologic treatment of voiding dysfunction. In: Urodynamics: Principles, Practice and Applications.. Edited by . Edinburgh: Churchill Livingstone1994. Google Scholar 32 : Terazosin: a review of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential in benign prostatic hyperplasia. Drug Aging1993; 3: 258. Google Scholar 33 : Calcium fluxes, calcium antagonists, and calcium related pathology in brain ischemia, hypoglycemia, and spreading depression: unifying hypothesis. J Cerebr Blood Flow Metab1989; 9: 127. Google Scholar 34 : Reperfusion of rat heart after brief ischemia induces proteolysis of calspectin (nonerythroid spectrin or fodrin) by calpain. Circ Res1995; 77: 603. Google Scholar 35 : Calcium channel antagonists prevent urinary bladder growth and neuroplasticity following mechanical stress. Amer J Physiol, part 21994; 266: R20. Google Scholar 36 : Efficacy and acceptability of tadenan (Pygeum africanum extract) in the treatment of benign prostatic hyperplasia (BPH): a multicentre trial in central Europe. Curr Med Res Opin1998; 14: 127. Google Scholar 37 : Comparison of phytotherapy (Permixon) with finasteride in the treatment of benign prostatic hyperplasia: a randomized international study of 1098 patients. Prostate1996; 29: 231. Google Scholar 38 : Placebo-controlled evaluation of the efficacy and tolerability of Permixon in benign prostatic hyperplasia after exclusion of placebo responders. Clin Drug Invest1996; 9: 291. Google Scholar 39 : Protective effect of Tadenan on bladder function secondary to partial outlet obstruction. J Urol1996; 155: 1466. Link, Google Scholar 40 : Beneficial effects of Tadenan therapy following two weeks of partial outlet obstruction in the rabbit. Neurourol Urodyn1997; 16: 583. Google Scholar 41 : Indigocarmine as a quantitative indicator of urothelial integrity. J Urol1991; 145: 842. Link, Google Scholar 42 : Trypan blue as an indicator of urothelial integrity. Neurourol Urodyn1990; 9: 269. Google Scholar From the Albany College of Pharmacy, Albany Medical College and Stratton Veterans Affairs Medical Center, Albany, New York, and Maruzen Pharmaceuticals, Onomichi City, Japan© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byOka M, Fukui T, Ueda M, Tagaya M, Oyama T and Tanaka M (2018) Suppression of Bladder Oxidative Stress and Inflammation by a Phytotherapeutic Agent in a Rat Model of Partial Bladder Outlet ObstructionJournal of Urology, VOL. 182, NO. 1, (382-390), Online publication date: 1-Jul-2009. Volume 167Issue 5May 2002Page: 2260-2266 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsplant extractsbladderrabbitsCa(2+)-transporting ATPasebladder neck obstructionMetricsAuthor Information ROBERT M. LEVIN Financial interest and/or other relationship with Maruzen and Laboratorie Debat. More articles by this author YOSHIHITO KAWASHIMA More articles by this author ROBERT E. LEGGETT More articles by this author CATHERINE WHITBECK More articles by this author PATRICK HORAN More articles by this author KENJI MIZUTANI More articles by this author Expand All Advertisement PDF downloadLoading ...