Artigo Revisado por pares

Platelet specific alloantigens on the platelet glycoprotein Ia/IIa complex

1989; Wiley; Volume: 72; Issue: 4 Linguagem: Inglês

10.1111/j.1365-2141.1989.tb04319.x

ISSN

1365-2141

Autores

James W. Smith, John G. Kelton, Peter Horsewood, Claudia Brown, Alan R. Giles, Ralph M. Meyer, Virgil L. Woods, Robert Burrows,

Tópico(s)

Blood disorders and treatments

Resumo

Summary The majority of platelet alloantigens are located on platelet glycoproteins IIb/IIIa. This report describes a codominant allelic system carried on the glycoprotein la/IIa complex, which we originally designated as Zav a /Zav b but which is identical to the Br a /Br b system. Furthermore Zav a was found to be identical to Hc a . The alloantigens could not be detected using a direct binding enzyme immunoassay (EIA) with intact platelets, but were readily detected using a glycoprotein capture EIA and by radioimmunoprecipitation techniques. The two index cases (designated as homozygous Zav a and Zav b ) had alloantibodies against the corresponding antigen and did not react with their own platelets. Using these alloantibodies and a monoclonal antibody that reacts with the platelet glycoprotein la/IIa complex (12F1). We demonstrated that all Ia/IIa molecules carry either Zav a or Zav b and we found that Zav a and Zav b are on discrete populations of Ia/IIa. Following immunodepletion using either anti‐Zav a or anti‐Zav b , all detectable Ia/IIa complexes from the respective homozygous platelets were removed. Immunodepletion of heterozygous Zav a /Zav b with either anti‐Zav a or anti‐Zav b did not reduce the amount of Ia/IIa complexes precipitable using the alternate alloantiserum. Population studies ( n = 50) indicated the phenotypic frequency of Zav a /Zav a is less than 1%; Zav a /Zav b is 18% and Zav b /Zav b is 82%. Four different alloantisera that had either anti‐Zav a or anti‐Zav b reactivity also carried reactivity against the Bak a or Bak b antigens which may suggest an association in the immune response to these alleles.

Referência(s)