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Comparative Study on Antibacterial Effect of Pediocin ACCEL and Nisin Against Fluorescencestained Listeria monocytogenes BCRC 14845

2005; Wiley; Volume: 70; Issue: 1 Linguagem: Inglês

10.1111/j.1365-2621.2005.tb09051.x

1750-3841

Chien‐Wei Wu, Li‐Jung Yin, Todd Hsu, Shann‐Tzong Jiang,

Food Quality and Safety Studies

Journal of Food ScienceVolume 70, Issue 1 p. M75-M80 Comparative Study on Antibacterial Effect of Pediocin ACCEL and Nisin Against Fluorescencestained Listeria monocytogenes BCRC 14845 Chien-Wei Wu, Chien-Wei Wu Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorLi-Jung Yin, Li-Jung Yin Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorTodd Hsu, Todd Hsu Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorShann-Tzong Jiang, Shann-Tzong Jiang Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this author Chien-Wei Wu, Chien-Wei Wu Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorLi-Jung Yin, Li-Jung Yin Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorTodd Hsu, Todd Hsu Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this authorShann-Tzong Jiang, Shann-Tzong Jiang Author Wu and Hsu are with Natl. Taiwan Ocean Univ., Keelung, Taiwan, ROC. Author Yin is with Dept. of Food Science, China College of Marine Technology and Commerce, Taipei, Taiwan, ROC. Author Jiangis with Dept. of Food and Nutrition, Providence Univ., 200 Chung-chi Rd., Shalu, Taichung County, Taiwan 433, ROC, and Dept. of Food Science, Natl. Taiwan Ocean Univ., Keelung, Taiwan 202, ROC. Direct inquiries to author Jiang (E-mail: [email protected]Search for more papers by this author First published: 31 May 2006 https://doi.org/10.1111/j.1365-2621.2005.tb09051.xCitations: 4AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract ABSTRACT: Effects of pediocin ACCEL (Class IIa, pediocin-like bacteriocins) and nisin on carboxyfluorescein diacetate (cFDA) -stained Listeria monocytogenes BCRC 14845 was carried out. Decrease of fluorescence intensity in stained cells and increase in efflux were observed with the increase in bacteriocin concentrations up to 12.5 μg/mL. No further decreases in fluorescence intensity and cell numbers were obtained when the concentration of bacteriocins was over 12.5μg/mL. The leakage of efflux was further confirmed by the release of intracel-lular ultraviolet-absorbing substances. Comparing the leaking time of fluorescence, the intensity of cFDA in nisin-treated cells within 1 min incubation was almost comparable to that in pediocin-treated samples after 40 min incubation. Considering the leakage of cFDA, UV-absorbing substances, changes in cell numbers during incubation of cFDA-stained cells, and restoration ability, nisin seemed to reveal mainly bacteriostatic effect and partially bactericidal effect on L. monocytogenes and to cause easier-to-repair membrane damage. However, pediocin ACCEL appeared to have both bacteriostatic and bactericidal effects on L. monocytogenes and to cause more difficult-to-repair membrane damage. References Abee T, Krockel L, Hill C. 1995. 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