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EBV, HCMV, HHV6, and HHV7 Screening in Bone Marrow Samples from Children with Acute Lymphoblastic Leukemia

2014; Hindawi Publishing Corporation; Volume: 2014; Linguagem: Inglês

10.1155/2014/548097

2314-6141

Abigail Morales‐Sánchez, Ericka N. Pompa‐Mera, Arturo Fajardo‐Gutiérrez, Francisco Javier Álvarez Rodríguez, Carolina Bekker-Méndez, José de Diego Flores-Chapa, Janet Flores‐Lujano, Elva Jiménez‐Hernández, José Gabriel Peñaloza‐González, M. C. Rodríguez-Zepeda, José Refugio Torres‐Nava, Martha Margarita Velázquez‐Aviña, Raquel Amador‐Sánchez, Martha Alvarado‐Ibarra, Nancy Carolina Reyes-Zepeda, Rosa Martha Espinosa‐Elizondo, María Luisa Pérez‐Saldivar, Juan Carlos Núñez-Enríquez, Juan Manuel Mejı́a-Aranguré, Ezequiel M. Fuentes‐Pananá,

Polyomavirus and related diseases

Acute lymphoblastic leukemia (ALL) is the most common cancer in childhood worldwide and Mexico has reported one of the highest incidence rates. An infectious etiology has been suggested and supported by epidemiological evidences; however, the identity of the involved agent(s) is not known. We considered that early transmitted lymphotropic herpes viruses were good candidates, since transforming mechanisms have been described for them and some are already associated with human cancers. In this study we interrogated the direct role of EBV, HCMV, HHV6, and HHV7 human herpes viruses in childhood ALL. Viral genomes were screened in 70 bone marrow samples from ALL patients through standard and a more sensitive nested PCR. Positive samples were detected only by nested PCR indicating a low level of infection. Our result argues that viral genomes were not present in all leukemic cells, and, hence, infection most likely was not part of the initial genetic lesions leading to ALL. The high statistical power of the study suggested that these agents are not involved in the genesis of ALL in Mexican children. Additional analysis showed that detected infections or coinfections were not associated with prognosis.