Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Vitamin D 3 Induces IDO + Tolerogenic DCs and Enhances Treg, Reducing the Severity of EAE

2013; Wiley; Volume: 19; Issue: 4 Linguagem: Inglês

10.1111/cns.12071

1755-5949

Alessandro S. Farias, Gabriela Salim Spagnol, Pedro Bordeaux‐Rego, Camila Ortolan Fernandes de Oliveira, Ana Gabriela M. Fontana, Rosemeire F.O. de Paula, Mariana P. A. Santos, Fernando Pradella, Adriel S. Moraes, Elaine C. Oliveira, Ana Leda F. Longhini, Alexandre César Santos de Rezende, Mauro Vaisberg, Leonilda Maria Barbosa dos Santos,

Vitamin D Research Studies

Summary Background A growing body of evidence supports the hypothesis that vitamin D is an important environmental factor in the etiology of T‐cell‐mediated autoimmune diseases such as multiple sclerosis ( MS ). Aim The purpose of this study was exploring the mechanisms underlying the beneficial effect of vitamin D3 in encephalomyelitis ( EAE ). Methods We treated monophasic experimental autoimmune EAE , induced in Lewis rat, with vitamin D3 and adoptively transfer tolerogenic bone marrow‐derived DC s generated in the presence of vitamin D3. Results This study provides evidence that the in vivo administration of vitamin D 3, as well as the adoptive transfer of vitamin D 3 ‐induced IDO + immature/tolerogenic dendritic cells, leads to a significant increase in the percentage of CD 4 + CD 25 + Foxp3 + regulatory T cells in the lymph nodes in a rat model of MS , experimental autoimmune EAE . Concomitant with the increase in this cell population, there is a significant decrease in the number of autoreactive T cells in the central nervous system. Bone marrow‐derived DC s cultivated in the presence of vitamin D 3 present a tolerogenic profile with high IL ‐10, TNF α, and IDO expression and decreased MHC ‐ II and CD 80 expression. The adoptive transfer of IDO + DC s induces a significant increase in the percentage of CD 4 + CD 25 + Foxp3 + T cells in the lymph nodes, comparable with vitamin D 3 treatment. Conclusion These mechanisms contribute actively to the generation of a microenvironment in the lymph nodes that suppresses the activation of encephalitogenic T cells, resulting in the downregulation of the inflammatory response in the central nervous system.