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Granulocyte-Macrophage and Macrophage Colony-Stimulating Factors Activate Intramacrophage Killing of Leishmania mexicana amazonensis

1990; Oxford University Press; Volume: 162; Issue: 1 Linguagem: Inglês

10.1093/infdis/162.1.224

1537-6613

J. L., Steven G. Reed, E. A. Wick, M Giordano,

Acute Lymphoblastic Leukemia research

Leishmania organisms are important pathogens, causing diseases worldwide. Standard therapies are often toxic and are not always effective. The effect of recombinant human granulocytemacrophage and macrophage colony-stimulating factors (GM-CSF and M-CSF) on intramacrophage survival of Leishmania mexicana amazonensis (Lma) were compared with those of interferon-γ (IFN-γ). Macrophages previously infected with Lma were treated with or without GM-CSF and M-CSF. Compared with no cytokine treatment, treatment with GM-CSF (0.1–100 ng/mI) or M-CSF (1:3.5 × l06-1:3.5 × 103 dilutions) caused a significant dose-dependent reduction in intracellular parasites, 427 ± 20 (mean ± SE) Lma/l00 macrophages. GM-CSF or MCSF in combination with IFN-γ resulted in more effective inhibition of intracellular parasites. Thus, the cytostatic activity appears to require interaction between cytokines, macrophages, and amastigotes. These cytokines are potential therapeutic agents for visceral leishmaniasis.