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Small Molecule Approaches for Promoting Ischemic Tissue Vascularization

2006; Lippincott Williams & Wilkins; Volume: 99; Issue: 3 Linguagem: Inglês

10.1161/01.res.0000236801.85717.7e

1524-4571

Federico Bussolino,

Ubiquitin and proteasome pathways

HomeCirculation ResearchVol. 99, No. 3Small Molecule Approaches for Promoting Ischemic Tissue Vascularization Free AccessEditorialPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toFree AccessEditorialPDF/EPUBSmall Molecule Approaches for Promoting Ischemic Tissue Vascularization Federico Bussolino Federico BussolinoFederico Bussolino From the Department of Oncological Sciences and Institute for Cancer Research and Treatment, Division of Molecular Angiogenesis, University of Torino, Candiolo, Italy. Originally published4 Aug 2006https://doi.org/10.1161/01.RES.0000236801.85717.7eCirculation Research. 2006;99:231–233Angiogenesis and arteriogenesis identify 2 specific processes that lead to the formation of new capillaries and the development of collateral vessels from pre-existing arterioles, respectively. Both processes characterize tissue repair and remodeling occurring in acute and chronic ischemic vascular diseases, and both processes represent the final targets of therapeutic angiogenesis aimed at providing an alternative treatment strategy for patients with lower limb ischemia and coronary artery disease.1,2Angiogenesis and arteriogenesis are driven by distinct, but partially overlapping, cellular and molecular pathways. Whereas the role of hypoxia and angiogenic inducers seems to be the trigger for pathological angiogenesis (ie, tumors, inflammatory chronic diseases, retinal maculopathy), fluid shear stress might be the most important stimulus for initiation of arteriogenesis.1,2 Besides these specific initial triggers, angiogenesis and arteriogenesis share growth factors, chemokines, proteases, inflammatory cells, and bone marrow-derived cells, which play quantitatively different roles in sustaining and refining these mechanisms of vascularization. A paradigmatic example of these differences is the differing roles played by inflammatory cells in angiogenesis and arteriogenesis. In fact, angiogenesis may also occur in the absence of leukocyte recruitment. On the contrary, fluid shear stress activates the expression of multiple genes in endothelial cells (EC) aimed mainly to trigger the attraction and adhesion of circulating blood cells, which are necessary and sufficient functions to maintain arteriogenesis.In the last 10 years, relevant strides have been made with respect to therapeutic angiogenesis. Proof-of-concept evidence for therapeutic growth factor, both gene and protein-mediated neovascularization, was provided in animal models of chronic myocardial and hindlimb ischemia. After encouraging results in human phase I trials using the prototypical growth factor families (vascular endothelial growth factors [VEGFs] and fibroblast growth factors [FGFs]), large randomized placebo-controlled phase II/III clinical trials have, however, yielded variable results. This limits our ability to draw firm conclusions.3 A second and more recent therapeutic approach has been supported by in vitro experiments and preclinical models demonstrating that after an ischemic injury, bone marrow mobilizes cell precursors, most probably of myeloid origin, which sustain angiogenesis and arteriogenesis by releasing soluble molecules around the nascent vessels and, to a minimal extent, by an incorporation in the newly formed vasculature.3,4 Even if the molecular and cellular bases of these observations are largely enigmatic, promising but not definitive conclusions come from trials using bone marrow–derived and peripheral blood-derived stem/progenitor cells in treatment of patients with acute myocardial infarction.3In this issue of Circulation Research, Murphy et al5 demonstrate that sokotrasterol sulfate, isolated from the marine sponge Topsentia ophirhaphidites, and a synthetic steroid analogue, 2β,3α,6α-cholestanetrisulfate, are powerful inducers of therapeutic angiogenesis in a murine hindlimb ischemia model. Murphy et al also show that sokotrasterol sulfate and 2β,3α,6α-cholestanetrisulfate activity strictly depends on sulfate residues. They clearly show that these 2 steroids are angiogenic in chick chorion allantoid membrane assay, and that the steroids generate stable and functional vessels in the ischemic tissues that persist after therapy interruption. Sokotrasterol sulfate induces in vitro proliferation, survival, and directional migration of EC. The specific upregulation of αvβ3 integrin, VEGF-A, and cyclooxygenase (COX)-2 are other features of the effect of this steroid on EC.5 The necessary role of these molecules in mediating the proangiogenic effect of sokotrasterol sulfate have been inferred by the use of specific inhibitors, thus allowing an hypothesis on its mechanism of action (Figure). Download figureDownload PowerPointPutative mechanism of action of sokotrasterol sulfate on EC. Sokotrasterol sulfate seems to be an indirect angiogenic inducer, which promotes vascularization by regulating transcription of some genes including vegf, cox-2, and integrin αv. Integrin αvβ3 cooperates with a VEGF-A–dependent autocrine loop occurring in angiogenic EC in promoting EC survival, motility, and proliferation. COX-2 is involved in a positive regulation of production and activity of VEGF-A and of αvβ3-dependent cell adhesion and migration. COX-2 metabolites may induce a proinflammatory environment, which could favor vascular arterialization (for details see text). Dotted arrows respectively indicates hypothetical activities.Integrin αβ heterodimers are major extracellular matrix (ECM) receptors that exist in different functional configurations with regard to their affinity for ligands. Integrin signaling is bidirectional. '"Inside-out" signals regulate integrin affinity for adhesive ligands, and ligand-dependent "outside-in" signals regulate cellular responses to adhesion.6 Integrins play key roles in embryonic and postnatal angiogenesis.7 Their crucial role is underlined by emerging data that, during vascular formation, the activity of endogenous proangiogenic factors is counterbalanced by the activity of endogenous antiangiogenic factors, such as secreted class 3 semaphorins, and that regulation of integrin adhesive functions represent a crucial shared target for both classes of factors.7 Historically, a large amount of data pointed to avβ3 integrin, a receptor for fibronectin, fibrin, and vitronectin as one the major players in blood vessel formation. Indeed, blockade of αvβ3 integrin with antagonists disrupts tumor and experimental angiogenesis.7 ECM of ischemic injured tissues is particularly enriched in the αvβ3 integrin ligands.8 Therefore, when activated by the proper ECM, αvβ3 integrin may exert a proangiogenic effect in at least 3 different ways: (1) by regulating adhesive and migratory processes; (2) by triggering antiapoptotic signals; and (3) by forming a supramolecular complex with VEGF receptor-2 that enhances its sensitivity to the proliferative and motility effect of VEGF-A.7 Notably, angiogenic EC produce growth factors, including VEGF-A.9 Therefore, the increase of αvβ3 integrin and VEGF-A by sokotrasterol sulfate may allow speculation that this steroid enhances the VEGF-A–mediated autocrine loop, which is further enhanced by αvβ3 integrin upregulation.The role of COX-2 in vessel formation has been established by connecting its prostanoid metabolites with VEGF-A production and action. COX-2 null cells do not produce VEGF-A, and COX-2 inhibitors prevent VEGF-A–mediated signals.10 On the other hand, VEGF-A increases COX-2 transcription.11 A more stringent link between the Murphy et al article5 and COX-2 activity in vascularization comes from the observation that COX-2 inhibition suppressed αvβ3-dependent EC spreading and migration.12 The molecular targets of COX-2 metabolites are Rac and cdcd42, 2 members of Rho family GTPases that regulate cytoskeleton dynamics. Furthermore, COX-2 metabolites, in particular prostaglandins, are known to directly and positively modulate αvβ3 activation.10The effect of sokotrasterol sulfate on EC migration and survival is also supported by the observation done by the same group of authors that this compound13 increases the expression of 14-3-3ζ cytosolic adaptor and cofilin, an actin regulatory protein, in EC. 14-3-3ζ promotes cell survival by sequestering and inactivating several proapoptotic proteins, including Bad and FOXO3a, after their phosphorylation by survival-inducing kinases such as Akt. Furthermore, 14-3-3ζ is phosphorylated by LIM kinase, which is implicated in the regulation of actin-depolymerizing factor/cofilin activity.14,15Murphy et al5 demonstrate that the vascular bed induced by sokotrasterol sulfate is functionally active. Despite the lack of histological studies and measurements of prostanoids and chemokines, the upregulation of COX-2 could indicate that sokotrasterol sulfate treatment is connected with an inflammatory condition, which in principle may favor vessel arterialization.1The effect of steroid in inhibiting and promoting vascularization has been well known for a long time. Angiogenic activity has been reported for ligands of the nuclear hormone receptor superfamily, such as androgens and estrogens. Recently, the molecular mechanism underlying angiogenic growth factors regulation by estrogens in the female reproductive tract has been elucidated by the finding that a functional estrogen responsive element is present in the promoter region of vegf.16 Therefore, the elucidation of transcriptome induced by sokotrasterol sulfate in EC will be of invaluable help to understand how this steroid acts on vasculature. In particular, the study of Murphy et al5 does not discriminate whether sokotrasterol sulfate induces the transcription of vegf, which in turn regulates that of COX-2 and the integrin function, or it has multiple activities with genes involved in angiogenesis. Transcriptome analysis will also bring insights on the acute and chronic potential adverse effects of the therapeutic use of sokotrasterol sulfate.The Murphy et al article5 gives a new and interesting perspective of the management of therapeutic angiogenesis by introducing the first example of the efficacy of a small and synthetic molecule in promoting revascularization in ischemic tissues. Target therapy based on small molecules seems to be a rational and positive evolution of studies founded on gene or protein delivery. Knowledge of small molecule chemical structure may facilitate pharmacokinetic parameters and cost reduction.The opinions expressed in this editorial are not necessarily those of the editors or of the American Heart Association.Sources of FundingThis study was supported by Associazione Italiana per la Ricerca sul Cancro, Ministero dell' Università e della Ricerca (MIUR) (60% and PRIN 2004 projects), Regione Piemonte and European Community (LSHM-CT-2003–503251) (http:://www.engv.org).DisclosuresNone.FootnotesCorrespondence to Federico Bussolino, MD, PhD, Institute for Cancer Research and Treatment, Department of Oncological Sciences, Division of Molecular Angiogenesis, Strada Provincale 142, Candiolo (Torino) 10060, Italy. E-mail [email protected] References 1 Carmeliet P. Mechanisms of angiogenesis and arteriogenesis. Nat Med. 2000; 6: 389–395.CrossrefMedlineGoogle Scholar2 Heil M, Schaper W. Influence of mechanical, cellular, and molecular factors on collateral artery growth (arteriogenesis). Circ Res. 2004; 95: 449–458.LinkGoogle Scholar3 Semenza GL. Therapeutic angiogenesis: another passing phase? Circ Res. 2006; 98: 1115–1116.LinkGoogle Scholar4 Zentilin L, Tafuro S, Zacchigna S, Arsic N, Pattarini L, Sinigaglia M, Giacca M. Bone marrow mononuclear cells are recruited to the sites of VEGF-induced neovascularization but are not incorporated into the newly formed vessels. Blood. 2006; 107: 3546–3554.CrossrefMedlineGoogle Scholar5 Murphy S, Larivée B, Pollet I, Craig KS, Williams DE, Huang X-H, Abbott M, Wong F, Curtis C, Conrads TP, Veenstra T, Puri M, Hsiang Y, Roberge M, Andersen RJ, Karsan A. Identification of sokotrasterol sulfate as a novel proangiogenic steroid. Circ Res. 2006; 99: 257–265.LinkGoogle Scholar6 Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell. 2002; 110: 673–687.CrossrefMedlineGoogle Scholar7 Serini G, Valdembri D, Bussolino F. Integrins and angiogenesis: a sticky business. Exp Cell Res. 2006; 312: 651–658.CrossrefMedlineGoogle Scholar8 Ju H, Dixon IM. Extracellular matrix and cardiovascular diseases. Can J Cardiol. 1996; 12: 1259–1267.MedlineGoogle Scholar9 Tang N, Wang L, Esko J, Giordano FJ, Huang Y, Gerber HP, Ferrara N, Johnson RS. Loss of HIF-1alpha in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis. Cancer Cell. 2004; 6: 485–495.CrossrefMedlineGoogle Scholar10 Ruegg C, Dormond O, Mariotti A. Endothelial cell integrins and COX-2: mediators and therapeutic targets of tumor angiogenesis. Biochim Biophys Acta. 2004; 1654: 51–67.MedlineGoogle Scholar11 Hernandez GL, Volpert OV, Iniguez MA, Lorenzo E, Martinez-Martinez S, Grau R, Fresno M, Redondo JM. Selective inhibition of vascular endothelial growth factor-mediated angiogenesis by cyclosporin A: roles of the nuclear factor of activated T cells and cyclooxygenase 2. J Exp Med. 2001; 193: 607–620.CrossrefMedlineGoogle Scholar12 Dormond O, Foletti A, Paroz C, Ruegg C. NSAIDs inhibit alpha V beta 3 integrin-mediated and Cdc42/Rac-dependent endothelial-cell spreading, migration and angiogenesis. Nat Med. 2001; 7: 1041–1047.CrossrefMedlineGoogle Scholar13 Karsan A, Pollet I, Yu LR, Chan KC, Conrads TP, Lucas DA, Andersen R, Veenstra T. Quantitative proteomic analysis of sokotrasterol sulfate-stimulated primary human endothelial cells. Mol Cell Proteomics. 2005; 4: 191–204.CrossrefMedlineGoogle Scholar14 Dougherty MK, Morrison DK. Unlocking the code of 14–3-3. J Cell Sci. 2004; 117: 1875–1884.CrossrefMedlineGoogle Scholar15 Soosairajah J, Maiti S, Wiggan O, Sarmiere P, Moussi N, Sarcevic B, Sampath R, Bamburg JR, Bernard O. Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin. EMBO J. 2005; 24: 473–486.CrossrefMedlineGoogle Scholar16 Hyder SM, Nawaz Z, Chiappetta C, Stancel GM. Identification of functional estrogen response elements in the gene coding for the potent angiogenic factor vascular endothelial growth factor. Cancer Res. 2000; 60: 3183–3190.MedlineGoogle Scholar Previous Back to top Next FiguresReferencesRelatedDetailsCited BySenzaki H (2008) Long-Term Outcome of Kawasaki Disease, Circulation, 118:25, (2763-2772), Online publication date: 16-Dec-2008. August 4, 2006Vol 99, Issue 3 Advertisement Article InformationMetrics https://doi.org/10.1161/01.RES.0000236801.85717.7ePMID: 16888247 Originally publishedAugust 4, 2006 KeywordssteroidsVEGFtherapeutic angiogenesisintegrincyclooxigenase-2PDF download Advertisement