Portal de Periódicos da CAPES

Study of oxidative stress induction after exposure to bisphenol a and methylparaben in rats

2011; Elsevier BV; Volume: 205; Linguagem: Inglês

10.1016/j.toxlet.2011.05.768

1879-3169

Anca Pop, Béla Kiss, Laurian Vlase, Daniela‐Saveta Popa, Ramona Păltinean, Rita Iepure, Felicia Loghin,

Carcinogens and Genotoxicity Assessment

Long exposure to small concentrations of xenoestrogens (XEs) seems to be responsible for the increase of certain hormone-dependent diseases. The results of the research concerning the study of potentially synergic interactions between XEs are few and contradictory and need further studies. The oxidative stress is involved in the generation of several pathologies, such as cancer. We studied the oxidative stress induction in rats after exposure to bisphenol A (BPA) and/or methylparaben, two xenobiotics identified as XEs. Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3-dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. The levels of MDA in rat plasma were determined by HPLCUV (detection at 307 nm) after derivatization with dinitrophenylhydrazine. 2,3-DHBA was analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode using an ion trap mass spectrometer with electrospray negative ionization. The ion transition monitored was m/z 153 → m/z (109). The results indicated that both studied xenobiotics have the potential of oxidative stress induction in rats but simultaneous exposure does not increase the intensity of effects. Rezumat Expunerea prelungita la concentraţii mici de xenoestrogeni (XE) pare a fi responsabila de cresterea incidenţei anumitor boli hormono-dependente. Rezultatele cercetarilor privind studiul unor potenţiale interacţiuni sinergice intre XE sunt puţine si contradictorii si necesita efectuarea unor noi investigaţii. Stresul oxidativ este implicat in generarea mai multor patologii, inclusiv a cancerului. S-a studiat inducerea stresului oxidativ la sobolani dupa expunere la bisfenol A (BPA) si/sau metilparaben, doua xenobiotice identificate ca fiind XE. Pentru evaluarea producerii stresului oxidativ s-a determinat concentrația plasmatica de malondialdehida (MDA), un produs final al peroxidarii lipidelor, si a acidului 2,3-dihidroxibenzoic (2,3-DHBA), un marker al