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Expression of plant genes during the development of pea root nodules

1985; Springer Nature; Volume: 4; Issue: 4 Linguagem: Inglês

10.1002/j.1460-2075.1985.tb03711.x

1460-2075

Francine Govers, Ton Gloudemans, Marja Moerman, Albert van Kammen, Ton Bisseling,

Plant Micronutrient Interactions and Effects

Article1 April 1985free access Expression of plant genes during the development of pea root nodules Francine Govers Francine Govers Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Ton Gloudemans Ton Gloudemans Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Marja Moerman Marja Moerman Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Albert van Kammen Albert van Kammen Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Ton Bisseling Ton Bisseling Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Francine Govers Francine Govers Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Ton Gloudemans Ton Gloudemans Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Marja Moerman Marja Moerman Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Albert van Kammen Albert van Kammen Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Ton Bisseling Ton Bisseling Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands Search for more papers by this author Author Information Francine Govers1, Ton Gloudemans1, Marja Moerman1, Albert Kammen1 and Ton Bisseling1 1Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands The EMBO Journal (1985)4:861-867https://doi.org/10.1002/j.1460-2075.1985.tb03711.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The expression of plant genes involved in the pea-Rhizobium symbiosis was studied by analysing mRNA from root nodules. The RNA was translated in vitro and the translation products were separated by two-dimensional gel electrophoresis. The results show differential expression of nodulin genes during root nodule development. One gene encoding N-40′ is expressed at a significant level 5 days before the leghemoglobin genes. Most other nodulin genes are expressed more or less concomitantly with the leghemoglobin genes whereas the N-21 mRNA is only present late during the development. In the development of ineffective root nodules induced by infection with different nod+fix− mutants of R. leguminosarum all nodulin genes are expressed except for the N-21 gene. The results suggest that neither bacteroid development, heme excretion nor nitrogen fixation are essential for the induction of nodulin gene expression in the host plant. Further, it appears that the amount of leghemoglobin in ineffective nodules is regulated at a post-transcriptional level. Previous ArticleNext Article Volume 4Issue 41 April 1985In this issue RelatedDetailsLoading ...