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MultiPLX: automatic grouping and evaluation of PCR primers

2004; Oxford University Press; Volume: 21; Issue: 8 Linguagem: Inglês

10.1093/bioinformatics/bti219

1367-4811

Lauris Kaplinski, Reidar Andreson, Tarmo Puurand, Maido Remm,

Genomics and Phylogenetic Studies

Summary: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer–primer interactions, primer–product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.