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Identification of a novel hemotropic mycoplasma in a splenectomized dog with hemic neoplasia

2004; American Veterinary Medical Association; Volume: 224; Issue: 12 Linguagem: Inglês

10.2460/javma.2004.224.1946

1943-569X

Jane E. Sykes, Nathan L. Bailiff, Louise M. Ball, Oded Foreman, Jeanne W. George, Michael M. Fry,

Rabies epidemiology and control

A 3-year-old sexually intact male Bull Mastiff underwent splenectomy for splenic thrombosis; prior to and after splenectomy, multiple blood transfusions were administered. Two weeks after the procedure, T-cell lymphoproliferative disease was diagnosed. Treatment with prednisone and chlorambucil was initiated, and 2 weeks later, cytologic examination of a blood smear revealed small (0.3 microm), coccoid basophilic bodies on the surface of approximately 70% of the RBCs. Morphologically, these resembled "Candidatus Mycoplasma haemominutum." A polymerase chain reaction assay was used to amplify a partial 16S rRNA sequence in blood obtained from the dog; the product was sequenced and compared with 16S rRNA gene sequences of other hemotropic mycoplasmas. The sequence was 98% homologous to that of "Candidatus M haemominutum", but only 77% homologous to that of M haemocanis and M haemofelis.