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Binding of Norwalk Virus-Like Particles to ABH Histo-Blood Group Antigens Is Blocked by Antisera from Infected Human Volunteers or Experimentally Vaccinated Mice

2002; American Society for Microbiology; Volume: 76; Issue: 23 Linguagem: Inglês

10.1128/jvi.76.23.12335-12343.2002

1098-5514

Patrick R. Harrington, Lisa C. Lindesmith, Boyd L. Yount, Christine L. Moe, Ralph S. Baric,

Viral gastroenteritis research and epidemiology

ABSTRACT Attachment of Norwalk (NV), Snow Mountain (SMV), and Hawaii (HV) virus-like particles (VLPs) to specific ABH histo-blood group antigens was investigated by using human saliva and synthetic biotinylated carbohydrates. The three distinct Norwalk-like viruses (NLVs) have various capacities for binding ABH histo-blood group antigens, suggesting that different mechanisms for NLV attachment likely exist. Importantly, antisera from NV-infected human volunteers, as well as from mice inoculated with packaged Venezuelan equine encephalitis virus replicons expressing NV VLPs, blocked the ability of NV VLPs to bind synthetic H type 1, Le b , and H type 3, suggesting a potential mechanism for antibody-mediated neutralization of NV.