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Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria

2002; American Society for Microbiology; Volume: 40; Issue: 11 Linguagem: Inglês

10.1128/jcm.40.11.4304-4307.2002

1098-660X

Sven Klaschik, Lutz Lehmann, Ansgar Raadts, Malte Book, Andreas Hoeft, Frank Stüber,

Mycobacterium research and diagnosis

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the opposite Gram probe.