HyPer-3: A Genetically Encoded H 2 O 2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging
2012; American Chemical Society; Volume: 8; Issue: 3 Linguagem: Inglês
10.1021/cb300625g
ISSN1554-8937
AutoresDmitry S. Bilan, Luke Pase, Linda Joosen, Andrey Yu. Gorokhovatsky, Yulia G. Ermakova, Theodorus W. J. Gadella, Clemens Grabher, Carsten Schultz, Sergey Lukyanov, Vsevolod V. Belousov,
Tópico(s)Mitochondrial Function and Pathology
ResumoHigh-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.
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