Produção Nacional
Revisado por pares
DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes
2009; Springer Science+Business Media; Volume: 7; Issue: 1 Linguagem: Inglês
10.1186/1476-8518-7-4
ISSN1476-8518
AutoresRubens R. Santos, Alexandrina Sartori, Deison Soares de Lima, Patricia Souza, Arlete Aparecida Martins Coelho‐Castelo, Vânia Luiza Deperon Bonato, Célio Lopes Silva,
Tópico(s)Immunotherapy and Immune Responses
ResumoOur group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases.In this investigation was evaluated the effect of a previous vaccination with DNA-HSP65 on diabetes development induced by Streptozotocin (STZ). C57BL/6 mice received three vaccine doses or the corresponding empty vector and were then injected with multiple low doses of STZ.DNA-HSP65 vaccination protected mice from STZ induced insulitis and this was associated with higher production of IL-10 in spleen and also in the islets. This protective effect was also concomitant with the appearance of a regulatory cell population in the spleen and a decreased infiltration of the islets by T CD8+ lymphocytes. The vector (DNAv) also determined immunomodulation but its protective effect against insulitis was very discrete.The data presented in this study encourages a further investigation in the regulatory potential of the DNA-HSP65 construct. Our findings have important implications for the development of new immune therapy strategies to combat autoimmune diseases.
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