Produção Nacional
Revisado por pares
A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
2015; Springer Nature; Volume: 46; Issue: 1 Linguagem: Inglês
10.1590/s1517-838246120130851
ISSN1678-4405
AutoresLeticia Mara Lima Angelini, Amanda Ribeiro Martins da Silva, Lucas de Freitas Coli Rocco, Cíntia D. F. Milagre,
Tópico(s)Pancreatic function and diabetes
ResumoA modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
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