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Molecular genetic variation within and among isolates of QPX (Thraustochytridae), a parasite of the hard clam Mercenaria mercenaria

2007; Inter-Research; Volume: 77; Linguagem: Inglês

10.3354/dao01848

1616-1580

Huimin Qian, Q. Liu, Bassem Allam, Jackie L. Collier,

Marine Bivalve and Aquaculture Studies

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 77:159-168 (2007) - DOI: https://doi.org/10.3354/dao01848 Molecular genetic variation within and among isolates of QPX (Thraustochytridae), a parasite of the hard clam Mercenaria mercenaria Hua Qian*, Qianqian Liu*, Bassem Allam, Jackie L. Collier** Marine Sciences Research Center, Stony Brook University, Stony Brook, New York 11794-5000, USA *These authors contributed equally to the work reported here**Email: jcollier@notes.cc.sunysb.edu ABSTRACT: The thraustochytrid known as QPX (Quahog Parasite Unknown) has sporadically caused disease in the hard clam Mercenaria mercenaria along the east coast of North America since the 1960s. We hypothesized that genetically distinct QPX strains might be responsible for outbreaks of QPX disease in different areas and tested this hypothesis by comparing several QPX isolates recovered from the recent outbreak in Raritan Bay, New York with QPX strains isolated from 2 outbreaks in Massachusetts, USA. There was no variation in small subunit rDNA (SSU rDNA), 5.8S rDNA, or 4 mitochondrial gene sequences. In contrast, both of the ribosomal ribonucleic acid (rRNA) operon intergenic spacers, internal transcribed spacers 1 and 2 (ITS1 and ITS2), revealed substantial sequence variation. However, strain-specific sequences were not detected because the ITS sequence variation within QPX isolates was comparable to the variation between isolates. ITS1 sequences recovered from an infected clam by amplification with a QPX ITS2-specific primer were identical to those recovered from the QPX isolates. KEY WORDS: Internal transcribed spacer 1 · Internal transcribed spacer 2 · SSU rRNA gene · 5.8S rRNA gene · Mitochondrial gene · Labyrinthulomycota Full text in pdf format PreviousNextCite this article as: Qian H, Liu Q, Allam B, Collier JL (2007) Molecular genetic variation within and among isolates of QPX (Thraustochytridae), a parasite of the hard clam Mercenaria mercenaria. Dis Aquat Org 77:159-168. https://doi.org/10.3354/dao01848Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 77, No. 2. Online publication date: September 14, 2007 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2007 Inter-Research.