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The informosome-like virus-specific ribonucleoprotein (vRNP) may be involved in the transport of tobacco mosaic virus infection

1984; Elsevier BV; Volume: 137; Issue: 1 Linguagem: Inglês

10.1016/0042-6822(84)90015-1

1096-0341

Yu. L. Dorokhov, N.M. Alexandrova, N.A. Miroshnichenko, J.G. Atabekov,

Legume Nitrogen Fixing Symbiosis

A new type of informosome-like virus-specific ribonucleoprotein (vRNP) differing from mature tobacco mosaic virus (TMV) particles in buoyant density and structure was found in TMV-infected cells (Yu. L. Dorokhov, N. M. Alexandrova, N. A. Miroshnichenko, and J. G. Atabekov, 1983, Virology 127, 237–252). Two groups of TMV ts mutants were used to discover whether there is a correlation between the vRNP formation and systemic spreading of virus infection (transport) over the infected plant. The first group of mutants (Ni118, flavum) contains a ts mutation in the coat protein gene but are capable of systemic spreading at nonpermissive temperature (tr transport); the second group of mutants (Ni2519, Ls1) cannot spread systemically at restrictive temperature (ts transport). It is shown that vRNP can be produced at restrictive temperature by tr-transport mutants but not by ts-transport mutants. The latter can produce vRNP only at a permissive temperature. The role of vRNP in long-distance transport of the virus infection is supported by two other observations: (a) upper leaves that were maintained at 5° accumulate potentially infective material and material with the properties of vRNP but not virus particles and (b) plants that were simultaneously infected with Lsl and Ni118 at a non-permissive temperature exhibited long-distance transport and vRNP. These results also implicate coat protein in long-distance transport. It is suggested that vRNPs are novel types of virus-specific particles that are involved in both cell-to-cell and long-distance transport of TMV infections.