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Polymerase I and transcript release factor (PTRF) regulates adipocyte differentiation and determines adipose tissue expandability

2014; Wiley; Volume: 28; Issue: 8 Linguagem: Inglês

10.1096/fj.14-251165

1530-6860

Sergio Pérez-Díaz, Lance A. Johnson, Robert M. DeKroon, José María Moreno‐Navarrete, Óscar Álzate, José Manuel Fernández‐Real, Nobuyo Maeda, José M. Arbones-Mainar,

Caveolin-1 and cellular processes

The FASEB JournalVolume 28, Issue 8 p. 3769-3779 Research CommunicationFree to Read Polymerase I and transcript release factor (PTRF) regulates adipocyte differentiation and determines adipose tissue expandability Sergio Perez-Diaz, Sergio Perez-Diaz Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Hospital Universitario Miguel Servet, Zaragoza, SpainSearch for more papers by this authorLance A. Johnson, Lance A. Johnson Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorRobert M. DeKroon, Robert M. DeKroon University of North Carolina Systems-Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Moreno-Navarrete, Jose M. Moreno-Navarrete Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigació Biomèdica de Girona (IdlBGi) Hospital Dr. Josep Trueta, Girona, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainSearch for more papers by this authorOscar Alzate, Oscar Alzate University of North Carolina Systems-Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Fernandez-Real, Jose M. Fernandez-Real Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigació Biomèdica de Girona (IdlBGi) Hospital Dr. Josep Trueta, Girona, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainSearch for more papers by this authorNobuyo Maeda, Nobuyo Maeda Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Arbones-Mainar, Corresponding Author Jose M. Arbones-Mainar jmarbones.iacs@aragon.es Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Hospital Universitario Miguel Servet, Zaragoza, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainCorrespondence: Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud, Hospital Universitario Miguel Servet, Zaragoza 50009, Spain. E-mail: jmarbones.iacs@aragon.esSearch for more papers by this author Sergio Perez-Diaz, Sergio Perez-Diaz Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Hospital Universitario Miguel Servet, Zaragoza, SpainSearch for more papers by this authorLance A. Johnson, Lance A. Johnson Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorRobert M. DeKroon, Robert M. DeKroon University of North Carolina Systems-Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Moreno-Navarrete, Jose M. Moreno-Navarrete Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigació Biomèdica de Girona (IdlBGi) Hospital Dr. Josep Trueta, Girona, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainSearch for more papers by this authorOscar Alzate, Oscar Alzate University of North Carolina Systems-Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Fernandez-Real, Jose M. Fernandez-Real Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigació Biomèdica de Girona (IdlBGi) Hospital Dr. Josep Trueta, Girona, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainSearch for more papers by this authorNobuyo Maeda, Nobuyo Maeda Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USASearch for more papers by this authorJose M. Arbones-Mainar, Corresponding Author Jose M. Arbones-Mainar jmarbones.iacs@aragon.es Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Hospital Universitario Miguel Servet, Zaragoza, Spain Centro de Investigación Biomédica en Red Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, SpainCorrespondence: Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud, Hospital Universitario Miguel Servet, Zaragoza 50009, Spain. E-mail: jmarbones.iacs@aragon.esSearch for more papers by this author First published: 08 May 2014 https://doi.org/10.1096/fj.14-251165Citations: 1 This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Impaired adipogenesis renders an adipose tissue unable to expand, leading to lipotoxicity and conditions such as diabetes and cardiovascular disease. While factors important for adipogenesis have been studied extensively, those that set the limits of adipose tissue expansion remain undetermined. Feeding a Western-type diet to apolipoprotein E2 knock-in mice, a model of metabolic syndrome, produced 3 groups of equally obese mice: mice with normal glucose tolerance, hyperinsulinemic yet glucose-tolerant mice, and prediabetic mice with impaired glucose tolerance and reduced circulating insulin. Using proteomics, we compared subcutaneous adipose tissues from mice in these groups and found that the expression of PTRF (polymerase I and transcript release factor) associated selectively with their glucose tolerance status. Lentiviral and pharmacologically overexpressed PTRF, whose function is critical for caveola formation, compromised adipocyte differentiation of cultured 3T3-L1cells. In human adipose tissue, PTRF mRNA levels positively correlated with markers of lipolysis and cellular senescence. Furthermore, a negative relationship between telomere length and PTRF mRNA levels was observed in human subcutaneous fat. PTRF is associated with limited adipose tissue expansion underpinning the key role of caveolae in adipocyte regulation. Furthermore, PTRF may be a suitable adipocyte marker for predicting pathological obesity and inform clinical management.—Perez-Diaz, S., Johnson, L. A., DeKroon, R. M., Moreno-Navarrete, J. M., Alzate, O., Fernandez-Real, J. M., Maeda, N., Arbones-Mainar, J. M. Polymerase I and transcript release factor (PTRF) regulates adipocyte differentiation and determines adipose tissue expandability. FASEB J. 28, 3769–3779 (2014). www.fasebj.org Citing Literature Supporting Information Filename Description fsb2028008043-sup-0001.pdfPDF document, 127.4 KB Supplementary material Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume28, Issue8August 2014Pages 3769-3779 RelatedInformation