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Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique

2009; Elsevier BV; Volume: 877; Issue: 24 Linguagem: Inglês

10.1016/j.jchromb.2009.06.022

1873-376X

Werner Neubauer, Armin König, Richard Bolek, Rainer Trittler, Monika Engelhardt, Manfred Jung, Klaus Kümmerer,

Drug-Induced Adverse Reactions

Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (λex: 261 nm, λem: 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1–5 mg/l using a 50 μl sample (r2 = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%.