On-line detection of bacterial adhesion in a shear gradient with bioluminescence by a Pseudomonas fluorescens (lux) strain
1992; Elsevier BV; Volume: 15; Issue: 1 Linguagem: Inglês
10.1016/0167-7012(92)90068-f
ISSN1872-8359
AutoresMarc W. Mittelman, Joan M. King, Gary S. Sayler, David C. White,
Tópico(s)Cell Image Analysis Techniques
ResumoOn-line detection of bacterial adhesion in a shear flow gradient was measured with an engineered bioluminescent bacterium. Parallel plate flow cells showed colonization by a Pseudomonas fluorescens strain containing the Vibrio fisheri (lux) bioluminescent operon. When induced by sodium salicylate, bulk phase and sessile cells were assayed quantitatively with an ammeter-photomultiplier-fiber optic system. The detection limit in a shear force gradient was 2×105 attached cells·cm−2 on glass coupons. Light production was found to correlate with biofilm lipid synthesis on a per unit area basis (1-14C-acetate incorporation). Light correlated directly with sessile bacterial acridine orange direct counts in the range of 105–107 cells·cm−2, providing on-line detection of both biofilm and bulk phase biomass and specific metabolic activity when induced.
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