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BIBTEX RIS

Development and Application of a Saccharomyces cerevisiae -Expressed Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Infectious Bronchitis Virus

2005; American Society for Microbiology; Volume: 43; Issue: 4 Linguagem: Inglês

10.1128/jcm.43.4.1982-1984.2005

ISSN

1098-660X

Autores

Aliandra M. Gibertoni, Maria de Fátima Silva Montassier, Janete Apparecida Desidério, Patrícia Emília Naves Givisiez, Cibele R. A. G. Furuyama, Helio José Montassier,

Tópico(s)

Viral Infectious Diseases and Gene Expression in Insects

Resumo

ABSTRACT A Saccharomyces cerevisiae -expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

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