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Influence of pH on the activity of chymotrypsin at a solid-liquid interface

1957; Elsevier BV; Volume: 68; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(57)90336-3

1096-0384

Angus McLaren, Eva F. Estermann,

Iron oxide chemistry and applications

A comparison of the action of an enzyme (chymotrypsin) on a substrate (denatured lysozyme) (a) in solution and (b) adsorbed on kaolinite, at a solid-liquid interface, reveals that the pH optimum for maximum activity is narrower and shifted to a higher pH with adsorbed substrate. This shift was predictable on the basis of the ionization properties of the kaolinite surfaces.