Immuno‐Raman microspectroscopy: In situ detection of antigens in tissue specimens by surface‐enhanced Raman scattering
2006; Wiley; Volume: 37; Issue: 7 Linguagem: Inglês
10.1002/jrs.1534
ISSN1097-4555
AutoresSebastian Schlücker, Bernd Küstner, A. Punge, R. Bonfig, Alexander Marx, Philipp Ströbel,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoAbstract We demonstrate the first realization of immuno‐Raman microspectroscopy, a novel methodology in immunohistochemistry. The in situ detection of antigens in tissue specimens is achieved by surface‐enhanced Raman scattering (SERS) from aromatic Raman labels covalently linked to the corresponding antibody. As an example of applications of this new Raman technique, we present the localization of prostate‐specific antigen (PSA) in epithelial tissue from patients with prostate carcinoma (PCA). Copyright © 2006 John Wiley & Sons, Ltd.
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