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First draft genome sequence of the Japanese eel, Anguilla japonica

2012; Elsevier BV; Volume: 511; Issue: 2 Linguagem: Inglês

10.1016/j.gene.2012.09.064

1879-0038

Christiaan V. Henkel, Ron P. Dirks, Daniëlle L. de Wijze, Yuki Minegishi, Jun Aoyama, Hans J. Jansen, Ben Turner, Bjarne Knudsen, Martin Bundgaard, Kenneth Lyneborg Hvam, Marten Boetzer, Walter Pirovano, Finn‐Arne Weltzien, Sylvie Dufour, Katsumi Tsukamoto, Herman P. Spaink, Guido E.E.J.M. van den Thillart,

Aquaculture Nutrition and Growth

The Japanese eel is a much appreciated research object and very important for Asian aquaculture; however, its genomic resources are still limited. We have used a streamlined bioinformatics pipeline for the de novo assembly of the genome sequence of the Japanese eel from raw Illumina sequence reads. The total assembled genome has a size of 1.15 Gbp, which is divided over 323,776 scaffolds with an N50 of 52,849 bp, a minimum scaffold size of 200 bp and a maximum scaffold size of 1.14 Mbp. Direct comparison of a representative set of scaffolds revealed that all the Hox genes and their intergenic distances are almost perfectly conserved between the European and the Japanese eel. The first draft genome sequence of an organism strongly catalyzes research progress in multiple fields. Therefore, the Japanese eel genome sequence will provide a rich resource of data for all scientists working on this important fish species.