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DAF-16 Target Genes That Control C. elegans Life-Span and Metabolism

2003; American Association for the Advancement of Science; Volume: 300; Issue: 5619 Linguagem: Inglês

10.1126/science.1083614

1095-9203

Siu Sylvia Lee, Scott Kennedy, Andrew C. Tolonen, Gary Ruvkun,

FOXO transcription factor regulation

Signaling from the DAF-2/insulin receptor to the DAF-16/FOXO transcription factor controls longevity, metabolism, and development in disparate phyla. To identify genes that mediate the conserved biological outputs of daf-2 /insulin-like signaling, we usedcomparative genomics to identify 17 orthologous genes from Caenorhabditis and Drosophila , each of which bears a DAF-16 binding site in the promoter region. One-third of these DAF-16 downstream candidate genes were regulatedby daf-2 /insulin-like signaling in C. elegans , and RNA interference inactivation of the candidates showed that many of these genes mediate distinct aspects of daf-16 function, including longevity, metabolism, anddevelopment.