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Suppression and Modulation of Cellular and Humoral Immune Responses to Haemophilus influenzae Type B (Hib) Conjugate Vaccine in Hib–Diphtheria‐Tetanus Toxoids–Acellular Pertussis Combination Vaccines: A Study in a Rat Model

2004; Oxford University Press; Volume: 191; Issue: 1 Linguagem: Inglês

10.1086/426396

1537-6613

Fatme Mawas, Gareth Newman, Samantha Burns, Michael J. Corbel,

Pneumonia and Respiratory Infections

We assessed a rat model to evaluate the immunogenicity of Haemophilus influenzae type b (Hib) conjugate vaccines and the effect on Hib immunogenicity of combining 2 Hib vaccines (Hib-tetanus toxoid [TT]-A and Hib-TT-B) with diphtheria-TT-acellular pertussis (DTaP)3 or DTaP5/inactivated poliovirus (IPV) vaccines. Rats were immunized subcutaneously with Hib alone or with Hib and DTaP-based vaccines; anti-Hib capsular polysaccharide IgG, poly-ribosyl-ribitol-phosphate (PRP), IgG subclass, and cellular immune responses were evaluated. Results showed a significant reduction in the antibody response to PRP when Hib-TT-A was administered in combination with DTaP3 and showed changes in the anti-PRP IgG subclass distribution between the separate and combination groups. However, combining Hib-TT-B with DTaP5/IPV did not reduce the anti- PRP antibody response. These results suggest that the model can predict the effect of combined administration of Hib and DTaP vaccines on Hib immunogenicity and would be suitable for preclinical studies of mechanisms of interference in Hib/DTaP vaccines.